Column : Shodex AFpak APG-894 (8.0mmID*50mm) Eluent : (A); 0.2M Sodium phosphate buffer(pH4.0) (B); 0.1M Sodium citrate buffer(pH2.5) Step gradient: (A) to (B) Flow rate : 1.0mL/min Detector : UV(280nm) Column temp. : Room temp.