Column : Shodex AFpak AHR-894 (8.0mmID*50mm) Eluent : (A); 0.01M Tris-HCl buffer(pH7.5) + 0.01M NaCl (B); 0.01M Tris-HCl buffer(pH7.5) + 0.3M NaCl Step gradient: (A) to (B) Flow rate : 1.0mL/min Detector : UV(280nm) Column temp. : Room temp.