Adenosine and Derivatives
Adenosine and derivatives were analyzed using Asahipak
GS-320 HQ (a multimode column).
Sample : 100micro-g/mL each, 20micro-L
1.
ATP
2.
ADP
3.
AMP
4.
Adenine
5.
Adenosine
Column : Shodex Asahipak GS-320 HQ (7.5mmID*300mm)
Eluent : 200mM NaH2PO4 aq./ 200mM H3PO4 aq.=500/62
Flow rate : 1.0mL/min
Detector : UV(260nm)
Column temp. : 30deg-C