Nucleotides (5)

Nucleotides were analyzed using AXpak WA-624 (a column for weak anion exchange chromatography).


Sample :
1. 5'CMP , 2. 5'TMP
3. 5'IMP , 4. 5'AMP
5. 5'GMP , 6. 3'GMP
7. 3'CMP , 8. 5'UDP
9. 5'ADP , 10. 5'CTP
11. 5'UTP , 12. 5'ATP
13. 5'GTP

Column       : Shodex AXpak WA-624 (6.0mmID*150mm)
Eluent       : (A); 0.1M NaH2PO4 aq./CH3CN=90/10
               (B); 0.5M NaH2PO4 aq./CH3CN=80/20
               Linear gradient: 0min to30min, (A) to (B)
Flow rate    : 1.0mL/min
Detector     : UV(260nm)
Column temp. : 60deg-C