Columns : Shodex Asahipak GS-710 7G (7.5mmID*500mm) x 2 Eluent : 50mM Tris-HCl buffer(pH7.2) + 300mM NaCl + 2mM EDTA-2Na Flow rate : 0.1mL/min Detector : UV(260nm) Column temp. : 30deg-C