Separation of Anomer
It has been reported that the anomer separation does not take place at high temperature.
Therefore, when using SUGAR series (
SP0810,
SC1011,
KS-801,
SZ5532 or
SC1211), set up the column
temperature at 70-80 deg-C.
Please refer to
Prevention of Anomer Separation.
Sample : 0.5% each, 10micro-L
Glucose,
Mannose,
Xylose,
Galactose,
Arabinose
Column : Shodex SUGAR SC1011 (8.0mmID*300mm)
Eluent : H2O
Flow rate : 0.7mL/min
Detector : Shodex RI
Column temp. : 29deg-C, 70deg-C