Column : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm) Fluent : (above); H3PO4/H2O/CH3CN=1/19/80 (below); H2O/CH3CN=20/80 Flow rate : 1.0mL/min Detector : Shodex RI Column temp. : (above); 50deg-C, (below); 30deg-C