Bread
Saccharides are extracted from bread using water and three saccharides, lactose, glucose and fructose are separated using 
SUGAR 
KS-801 (a column for saccharides analysis). There is a unknown peak after the peak of fructose. 
It is recommended to use a guard column for the analysis of actual sample which may contain impurities.

Sample : 
Bread
1. 
Lactose
2. 
Glucose
3. 
Fructose
Columns      : Shodex SUGAR KS-G (6.0mmID*50mm) + KS-801 (8.0mmID*300mm)
Eluent       : H2O
Flow rate    : 1.0mL/min
Detector     : Shodex RI
Column temp. : 80deg-C