Saccharides in powdered Milk

High sensivity analysis of saccharides in powdered milk was performed using Asahipak NH2P-50 4E (a column for saccharides analysis) and an electrochmical detection system.


Sample : powdered Milk
1. Lactulose
2. Lactose

Instrument        : Agilent 1050 system
Reagent           : 0.6M LiOH 0.8mL/min
Mode              : pulse
Working electrode : Gold
Potential         : Pot 1=0.65V, Pot 2=-0.95V, Pot=0.15V

Column       : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm)
Eluent       : (A); CH3CN/H2O=80/20
               (B); H2O
                0min to 40min, 5% (B) to 40% (B)
               40min to 45min, 40% (B)
Flow rate    : 1.0mL/min
Detector     : Agilent 1049A Electrochemical
Column temp. : 40deg-C