Yogurt: Sugar added (SP0810)
Sugar added yogurt was analyzed using SUGAR
SP0810.
(Sample pretreatment)
1) Take 5g of sugar added yogurt in a beaker, add 30mL of pure water and neutralize
with 10%(w/v) NaOH.
2) Apply ultrasonic vibration for 30 minutes and make 50 mL solution.
3) Filtrate with No.5B paper filter and then with 0.45 micron membrane
filter.
Sample :
Yogurt, 5micro-L
1.
Sucrose
2.
Lactose
3.
Glucose
4.
Galactose
Column : Shodex SUGAR SP0810 (8.0mmID*300mm)
Eluent : H2O
Flow rate : 0.6mL/min
Detector : Shodex RI
Column temp. : 85deg-C