Chitosan-Oligosaccharides (Chitoorigosaccharides) (1)
Chitosan-oligosaccharides are oligosaccharides which have the structure that D-glucosamines
are bonded by beta-1,4 bonding and physiologically acitive. From dimer to hexamer of chitosan-oligosaccharide are separated using Asahipak
NH2P-50 4E,
a column for sugar analysis and evaporative light scattering detector (ELSD).
Sample :
Mixture of Chitosan-oligosaccharides (Chitooligosaccharides)
(Seikagaku Co. code:800105 Chitosan-Oligosaccharides)
1.
D-Glucosamine
2. Chitobiose: dimer
3. Chitotriose: trimer
4. Chitotetraose: tetramer
5. Chitopentaose: pentamer
6. Chitohexaose: hexamer
Column : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm)
Eluent : CH3CN/H2O=70/30
Flow rate : 1.0mL/min
Detector : ELSD
Column temp. : 30deg-C