Analysis of Mannitol according to USP Method (MN-431)

According to USP method (United States Pharmacopeia 23), mannitol should be analyzed using a column which can separate mannitol and and sorbitol with R (resolution) of equal to or greater than 2.0. USPpak MN-431 is a column specially designed for mannitol analysis, whcih satisfies this criterion.


Sample :
1. Mannitol
2. Sorbitol

Column       : Shodex USPpak MN-431 (4.0mmID*250mm)
Eluent       : H2O
Flow rate    : 0.5mL/min
Detector     : Shodex RI
Column temp. : 60deg-C

The measurement of mannitol by USP method should be done at 30 to 85 deg-C within 2 deg-C.