Analysis of Pinitol (DC-613)
Pinitol, a compound present in pine and legumes, is a methylester of
chiro-inositol (an optical isomer of
myo-inositol) effective in lowering blood glucose levels.
For this reason, it is sold in combination with myo-inositol (a compound known for inhibiting fat storage) as a diet supplement for treating metabolic syndrome.
Here, RSpak
DC-613 columns for sugar analysis were used here to analyze pinitol and
myo-inositol,
chiro-inositol simultaneously with mono- and disaccharides.
Sample : 0.1% each, 10micro-L
1.
Pinitol
2.
Fructose
3.
Glucose
4.
Sucrose
5.
chiro-Inositol
6.
myo-Inositol
Column : Shodex RSpak DC-613 (6.0mmID*150mm)
Eluent : H2O/CH3CN=25/75
Flow rate : 1.0mL/min
Detector : Shodex RI
Column temp. : 70deg-C