Hyaluronic Acid (3) (KS-807 vs SB-806 HQ)

The analytical results of hyaluronic acid are shown. In the analysis with OHpak SB-806 HQ (exclusion limit : 2.0x10⁷g/mol), although HA 30 is larger than HA 20 by 10⁶ in MW, both of them elute around exclusion limit. On the other hand, SUGAR KS-807 (exclusion limit : 2.0x10⁸g/mol) anables us to distinguish them clearly.


Sample : Hyaluronic acid, 100micro-L
0.028% HA 30 (MW:3.0x 10⁶)
0.026% HA 20 (MW:2.0x10⁶)
0.027% HA 12 (MW:1.2x10⁶)
(offered by Shiseido Company, Limited)

Sample : Hyaluronic acid, 100micro-L
0.028% HA 30 (MW:3.0x10⁶)
0.026% HA 20 (MW:2.0x10⁶)
0.027% HA 12 (MW:1.2x10⁶)
(offered by Shiseido Company, Limited)

(above) 
Column       : Shodex OHpak SB-806 HQ (8.0mmID*300mm)
Eluent       : 0.2M NaCl aq.
Flow rate    : 0.5mL/min
Detector     : Shodex RI & MALS(Multi angle laser light scattering detector)
Column temp. : Room temp.
  
(below)
Column       : Shodex SUGAR KS-807 (8.0mmID*300mm)
Eluent       : 0.2M NaCl aq.
Flow rate    : 0.5mL/min
Detector     : Shodex RI & MALS(Multi angle laser light scattering detector)
Column temp. : Room temp.