p-Nitrophenyl-galactoside, -glucoside and -mannoside

The ligand of AFpak ACA-894 column is concanavalin A which can distinguish sugars such as mannoisde and glucoside. The column is suited to high-selective analysis and fractionation of saccharides or glycoproteins.


Sample :
1. p-Nitrophenyl-alpha-D-galactoside
2. p-Nitrophenyl-alpha-D-glucoside
3. p-Nitrophenyl-alpha-D-mannoside

Column       : Shodex AFpak ACA-894 (8.0mmID*50mm)
Eluent       : 0.02M Tris-HCl buffer(pH7.4) + 0.25M NaCl
Flow rate    : 1.0mL/min
Detector     : UV(280nm)
Column temp. : Room temp.