| Substance | Elution Volume (mL) | |
|---|---|---|
| 3mM HClO4 aq./CH3CN =90/10 |
3mM HClO4 aq. | |
| Oxalic acid |
9.28
|
9.62
|
| Maleic acid |
9.91
|
10.51
|
| alpha-Ketoglutaric acid |
10.39
|
10.87
|
| Citric acid |
10.99
|
11.71
|
| Isocitric acid |
11.05
|
11.88
|
| 2-Ketogluconic acid |
11.18
|
11.52
|
| Glucuronic acid |
11.29
|
11.71
|
| Oxalacetic acid |
11.40
|
11.77
|
| Pyruvic acid |
11.42
|
11.76
|
| Phosphoric acid |
11.43
|
11.27
|
| Tartaric acid |
11.53
|
12.14
|
| trans-Aconitic acid |
11.57
|
13.11
|
| Galacturonic acid |
11.90
|
12.41
|
| Malonic acid |
12.10
|
12.96
|
| Gluconic acid |
12.24
|
12.78
|
| Malic acid |
12.37
|
13.37
|
| Glyoxylic acid |
12.70
|
13.26
|
| alpha-Hydroxygltaric acid |
12.71
|
14.02
|
| Ascorbic acid |
12.71
|
13.88
|
| Succinic acid |
13.77
|
15.79
|
| Itaconic acid |
14.08
|
16.80
|
| Fumaric acid |
14.14
|
17.16
|
| Uric acid |
14.99
|
18.31
|
| Glycolic acid |
15.12
|
16.26
|
| Adipic acid |
15.16
|
20.17
|
| Lactic acid |
15.23
|
16.67
|
| 2-Hydroxyisobutyric acid |
15.26
|
17.05
|
| Mesaconic acid |
15.42
|
20.47
|
| beta-Hydroxypropionic acid |
15.61
|
17.18
|
| Levulinic acid |
16.44
|
20.27
|
| Formic acid |
16.59
|
17.86
|
| Acetic acid |
17.32
|
19.41
|
| Pyroglutamic acid |
18.02
|
20.99
|
| Propionic acid |
18.82
|
22.54
|
| Isobutyric acid |
20.13
|
25.59
|
| n-Butyric acid |
20.87
|
27.61
|
| Isovaleric acid |
22.16
|
32.28
|
| CO32-,HCO3- |
23.36
|
24.50
|
| n-Valeric acid |
24.64
|
38.89
|
Columns : Shodex RSpak KC-811 (8.0mmID*300mm) x 2 Eluent : 3mM HClO4 aq./CH3CN=90/10, 3mM HClO4 aq. Flow rate : 1.0mL/min Column temp. : 50deg-C