Elution Volume of Aromatic Organic Acids

RSpak NN-814, which has a higher polarity gel than that of KC-811 is suitable for the analysis of aromatic organic acids. The ion exclusion and reversed phase modes contribute to the separation of aromatic organic acids.

Substance	Elution Volume (mL)
Shikimic acid	10.1
DL-Mandelic acid	19.2
Phthalic acid	19.8
Trimellitic acid	23.0
m-Hydroxybenzonic acid	46.5
Vanillic acid	47.1
p-Hydroxybenzoic acid	47.6
o-Hydroxybenzoic acid	48.8
3-Phenylpropionic acid	49.7

Substance	Elution Volume (mL)
Benzoic acid	51.0
Caffeic acid	66.9
Phenylpropionic acid	68.4
Trimesic acid	69.0
Salicylic acid	71.4
m-Toluic acid	86.4
Ferulic acid	87.0
p-Coumaric acid	90.3

Column       : Shodex RSpak NN-814 (8.0mmID*250mm)
Eluent       : 0.1% H3PO4 aq.
Column temp. : 60deg-C