Columns : Shodex RSpak KC-LG (8.0mmID*50mm) + KC-811 (8.0mmID*300mm) x 2 Eluent : 3mM HClO4 Reagent : 10-fold diluted ST3-R (for post-column method) Flow rate : (Eluent); 1.0mL/min, (Reagant); 0.7mL/min Detector : VIS(430nm) Column temp. : 40deg-C