Columns : Shodex RSpak KC-LG (8.0mmID*50mm) + DE-613 (6.0mmID*150mm) x 2 + KC-811 (8.0mmID*300mm) x 3 Eluent : 2mM HClO4 aq. Reagent : 10-fold diluted ST3-R (for post-column method) Flow rate : (Eluent); 1.0mL/min, (Reagent); 0.5mL/min Detector : VIS(430nm) Column temp. : 50deg-C