Columns : Shodex RSpak KC-LG (8.0mmID*50mm) + KC-811 (8.0mmID*300mm) x 2 Eluent : 4.8mM HClO4 Reagent : 10-fold diluted ST3-R (for post-column method) Flow rate : (Eluent); 1.0mL/min, (Reagent); 1.1mL/min Detector : VIS(430nm) Column temp. : 63deg-C