Scopolamine and Atropine

Scopolamine and atropine were analyzed using Asahipak ODP-50 4D (a column for reversed phase chromatography).
Please refer to Analysis of Short Amines without Using Ion Pair Reagent.


Sample :
1. Scopolamine
2. Atropine 

Column       : Shodex Asahipak ODP-50 4D (4.6mmID*150mm)
Eluent       : 50mM Phosphate buffer(pH10)/CH3CN=70/30
Flow rate    : 0.6mL/min
Detector     : UV(210nm)
Column temp. : 30deg-C
 
: ODS (other manufacturer 4.6mmID*150mm)
: 0.1% Sodium 1-pentanesulfonate/CH3CN=60/40
: 1.4mL/min
: UV(210nm)
: 60deg-C