Phenobarbital in Serum

Phenobarbital in serum was analyzed using a column for rapid analyses of medicines, MSpak GF-310 4B. First, large molecular weight portion, serum proteins was eluted, and then, phenobarbital which had interaction of adsorption and partition with packing material was eluted.


Sample : 2micro-L
5micro-L/mL Phenobarbital in Control serum I Wako
(from Wako Chemicals Indusries, Ltd.)

Column       : Shodex MSpak GF-310 4B (4.6mmID*50mm) 
Eluent       : 50mM CH3COONH4/CH3CN=70/30
Flow rate    : 0.25mL/min
Detector     : UV(220nm)
Column temp. : 30deg-C