Analysis of VMA and Creatinine using Column Switching Method

Vanillylmandelic acid (VMA) and creatinine in urea are determined by fluorescent detection using three columns, Asahipak ES-502N, GS-320 and NH2P-50 and column-switching method. GS-320 7E is an old type of GS-320 HQ.

Sample : a. Diluted urine , b. Standard VMA and Creatinine

Columns      : Shodex Asahipak ES-502N 7C (7.5mmID*100mm), GS-320 7E (7.5mmID*250mm), NH2P-50 4E (4.6mmID*250mm)
Eluent       : 0.35mol Propionic acid + 0.3mol NaOH in 1kg H2O(pH3.75)
Flow rate    : 0.7mL/min
Detector     : Fluorescence detector(Ex: 280nm, Em: 315nm)

Courtesy of Dr. Seki, Osaka University