Hippuric Acid etc. in Urine (1)

Some metabolites in urine have been added to the health check as special items in Japan recently. From the items, hippuric acid, methylhippuric acid (o-, m-, p-) and mandelic acid which are metabolites of toluene, xylene and styrene and creatinine were analyzed using ODSpak F-411 (a column for reversed phase chromatography) within 10 minutes.


Sample :
1. Mandelic acid
2. Hippuric acid
3. o-Methylhippuric acid
4. p-Methylhippuric acid
5. m-Methylhippuric acid
6. Creatinine

Column      : Shodex ODSpak F-411 (4.6mmID*150mm)
Eluent      : [CH3CN/Buffer=12/88]+4.5mM sodium dodecyl sulfate
              (Buffer: 20mM H3PO4+20mM beta-Cyclodextrin (pH2.5))
Flow rate   : 1.0mL/min
Detector    : UV(225nm)
Colomn temp.: 50deg-C