Analysis of Polyamines by On-Column Derivertization

Polyamines were analyzed by on-column derivertization using Asahipak ODP-50 4D.
Refer to Instruments for On-Column Derivetization



Sample : 10micro-L
Histamine(Him) , Diethylenetriamine(Det:I.S.) , Tyramine(Tym) , 1,3-Diaminopropane(Dap)
Putrescine(Put) , Spermidine(Spd) , Spermine(Spm) , Cadaverine(Cad)

Column       : Shodex Asahipak ODP-50 4D (4.6mmID*150mm)
Eluent       : 50mM Borate buffer (pH9.9) - CH3CN(77:23,v/v)
               containing 2mM OPA(o-Phthalaldehyde) and 2mM NAC(N-Acetyl-L-cysteine)
Flow rate    : 0.5mL/min
Detector     : Fluorescence(Ex. 330nm, Em. 430nm)
Column temp. : 40deg-C

K.Saito, M.Horie, N.Nose,K.Nakagomi and H.Nakazawa, Anal. Sci. 8,675(1992)