Water-Soluble Vitamins (3)

Compared with the analysis using an ion pair reagent, isocratic analysis can be easily carried out by using the RSpak NN-814 column with a buffer eluent containing some organic solvent. This is a new analysis method, which does not require an ion pair reagent. The NN-814 is a polymer-based reversed phase column containing a small amount of sulfo groups.


Sample :
1. Pantothenic acid
2. Orotic acid (Vitamin B₁₃)
3. Pyridoxine HCl (Vitamin B₆)
4. Nicotinamide
5. Vitamin B₁
6. Vitamin B₂

Column       : Shodex RSpak NN-814 (8.0mmID*250mm)
Eluent       : 45mM KH2PO4 + 55mM Na2HPO4 + 7% CH3OH
Flow rate    : 1.0mL/min
Detector     : UV(210nm)
Column temp. : Room temp.