Water-Soluble Vitamins (4)

Six vitamines and caffein were separated isocratically using RSpak DM-614.


Sample :
1. Vitamin C
2. Vitamin B₁₃
3. Vitamin B₆
4. Vitamin B₁
5. Vitamin B₃
6. Caffeine

Column       : Shodex RSpak DM-614 (6.0mmID*150mm)
Eluent       : 0.055M Na2HPO4 + 0.045M KH2PO4 aq.
Flow rate    : 1.0mL/min
Detector     : UV(254nm)
Column temp. : 28deg-C