Food Containing Vitamins

Vitamins in food were analyzed using RSpak DE-413 ( a column for reversed phase chromatography ).

	Concentration	Contents in 4g
1. Vitamin C	145.8micro-g/mL	87.5mg
2. Vitamin B6	21.2micro-g/mL	12.7mg
3. Vitamin B2	7.3micr-og/mL	4.4mg

Column       : Shodex RSpak DE-413 (4.6mmID*150mm)
Eluent       : (A); 1/30M Potasium phosphate buffer(pH6.8)/CH3CN=98/2
               (B); H2O/CH3CN=50/50
               gradient: 0min to 4min, 100% (A), 
                         4min to 15 min, 100% (A) to 30% (A), 
                         After 15 min, 30% (A) 
Flow rate    : 0.6mL/min
Detector     : UV(254nm)
Column temp. : 35deg-C