Simultaneous Analysis of Vitamin E Homologs

Naturally occurring Vitamin E is classified into two categories: tocopherols and tocotrienlols, each containing 4 subcategories, and therefore amounting to a total of 8 homologs. Tocopherols exhibit a higher bioactivity, the most active type being α-tocopherol, and for this reason the term vitamin E generally refers to α-tocopherol. In this application, the silica 5SIL 4D normal phase column was used to remove β-tocophenol and analyze simultaneously 7 kinds of tocopheros and tocotrienols.


Sample : Vitamin E, 20micro-L
1. α-Tocopherol 5μg/mL
2. α-Tocotrienol 10μg/mL
3. β-Tocopherol 5μg/mL
4. γ-Tocopherol 5μg/mL
5. γ-Tocotrienol 10μg/mL
6. δ-Tocopherol 5μg/mL
7. δ-Tocotrienol 10μg/mL

Column       : Shodex Silica 5SIL 4D (4.6mmID*150mm)
Eluent       : n-Hexane/Isopropanol/CH3COOH=1000/6/5                   
Flow rate    : 1.0mL/min
Detector     : Fluorescence(Ex. 298nm, Em. 325nm)
Column temp. : 30deg-C