Column : Shodex Asahipak ODP-50 6D (6.0mmID*150mm) Eluent : (A); 0.1M H3PO4(pH2.0) (B); 0.1M H3PO4(pH2.0)/CH3CN=70/30 Linear gradient: 0min to 10min, (A) afetr 10min, 2.5%/min gradient of (B) Flow rate : 0.8mL/min Detector : UV(280nm)