Steroides

Three kinds of steroid were separated using OHpak SB-802.5 HQ (a column for GFC separation).


Sample : 0.01% each, 20micro-L
1. Cortisone
2. Prednisolone
3. Testosterone

Column       : Shodex OHpak SB-802.5 HQ (8.0mmID*300mm)
Eluent       : H2O/CH3CN=60/40
Flow rate    : 1.0mL/min
Detector     : UV(240nm)
Column temp. : 70deg-C