Calibration Curve for LF-804 (DMSO/DMF Eluent: Pullulan)

Different kinds of pullulan were separated using organic SEC (GPC) column GPC LF-804. DMSO/DMF eluent is sometimes necessary to analyse starch. In this case, linear type LF-804 is effective.


Sample : 100micro-L
Pullulan (MW:1,600,000) 0.05%
Pullulan (MW:788000) 0.1%
Pullulan (MW:404,000) 0.1%
Pullulan (MW:212,000) 0.1%
Pullulan (MW:112,000) 0.1%
Pullulan (MW:47,300) 0.1%
Pullulan (MW:22,800) 0.1%
Pullulan (MW:11,800) 0.1%
Pullulan (MW:5,900) 0.1%
Maltotriose (MW:504) 0.1%
Maltose (MW:342) 0.1%
Glucose (MW:180) 0.1%

Column       : Shodex GPC LF-804 (8.0mmID*300mm)
Eluent       : 10mM LiBr in DMSO/DMF=75/25
Flow rate    : 0.6mL/min
Detector     : Shodex RI
Column temp. : 50deg-C