Effect of Flow Rate on Elution Pattern

Polystyrene standard (Mw: 500) was analyzed using GPC KF-802 (a column for organic SEC (GPC)). For improving resolution, the appropriate flow rate should be selected considering the diffusion speed. In this case, a higher resolution can be realized with a lower flow rate. Although a reduction of the flow rate from 2.0mL/min to 1.0mL/min brought about a large difference in resolution, a reduction from 1.0mL/min to 0.5mL/min did not. Considering the time for analysis, the use of 1.0mL/min is recommended for the analysis of the sample.


Sample : PS, Polystyrene 500

Column       : Shodex GPC KF-802 (8.0mmID*300mm) 
Eluent       : THF
Flow rate    : (A)2.0mL/min
               (B)1.0mL/min
               (C)0.5mL/min
Detector     : UV(254nm)
Column temp. : Room temp.