Comparison of Chromatogram of PEG Mixtures
The polymer-based OHpak
SB-803 HQ column has a large distribution
of pore size. So, SB-803 HQ is suitable for the measurement of the synthetic polymer
with large molecular weight distribution. The silica-based PROTEIN
KW-803
and
KW-802.5 columns are suitable for the analysis of protein, because
a distribution of pore size of them is comparatively narrow.
Sample :
1.
Poly(ethylene glycol) (MW: 20,000)
2.
Poly(ethylene glycol) (MW: 4,000)
3.
Poly(ethylene glycol) (MW: 1,000)
4.
Poly(ethylene glycol) (MW: 400)
5.
EG, Ethylene glycol (MW: 62)
Columns : Shodex OHpak SB-803 HQ or PROTEIN KW-802.5 or KW-803 (8.0mmID*300mm each)
Eluent : H2O
Flow rate : 0.5mL/min
Detector : Shodex RI
Column temp. : 30deg-C