Molecular Weight Distribution of Gelatin Extract (1)

Gelatin contains alpha, beta and gamma chains, and the ratio of their content is said to have a significant effect on the performance of gelatin. The Asahipak GS-620 HQ column is suitable for general analysis of the components of gelatin and Asahipak GS-620 7G-P is especially suitable for analysis of the components of gelatin by the PAGI method(test method of gelatin for photo film in Japan). When using an Asahipak GS-620 7G-P, the column should be pretreated in accordance with "Precautions in Analyzing Gelatin" attached to the operation manual so as to obtain reproducible data.

Sample : Gelatin

 
Column       : Shodex Asahipak GS-620 7G-P (7.5mmID*500mm) x 2 
Eluent       : 0.2M Sodium phosphate(pH7.0) 
Flow rate    : 0.3mL/min 
Detector     : UV(220nm) 
Column temp. : 30deg-C 

Courtesy of Dr. Mizusawa and Dr. Ohno, Chiba University