Molecular Weight Distribution of Gelatin Extract (2)

Gelatin contains alpha, beta and gamma chains, and the ratio of their content is said to have a significant effect on the performance of gelatin. The Asahipak GS-620 HQ column is suitable for general analysis of the components of gelatin and Asahipak GS-620 7G-P is especially suitable for analysis of the components of gelatin by the PAGI method(test method of gelatin for photo film in Japan). When using a Asahipak GS-620 7G-P, the column should be pretreated in accordance with "Precautions in Analyzing Gelatin" attached to the operation manual so as to obtain reproducible data.


Sample : Gelatin

Columns      : Shodex Asahipak GS-620 7G-P (7.5mmID*500mm) x 2
Eluent       : 0.2M NaH2PO4(pH6.8)
Flow rate    : 0.57mL/min
Detector     : UV(220nm)
Column temp. : 50deg-C

Courtesy of Dr. Ohno, Chiba University