Hydrophile-Lipophile Balance (HLB)
Hydrophile-Lipophile Balance (HLB) was analyzed using Asahipak 
GS-310 7G 
( a multisolvent GPC column ). HLB is one of the indicators representing the ratio 
of the hydrophilicity of a surfactant to its hydrophobicity. The HLB value and 
elution volume of octylphenol ethoxylate with a different polymerization degree 
show a good correlation. Therefore, it is possible to measure the HLB value from 
the elution volume. 
GS-310 7G is an old type of GF-310 HQ.

Sample : 
Octylphenol ethoxylate
Column       : Shodex Asahipak GS-310 7G (7.5mmID*500mm)
Eluent       : H2O/CH3CN=50/50
Flow rate    : 1.0mL/min
Detector     : UV(280nm)
Column temp. : 30deg-C