Nonionic and Ionic Surfactants (2)

Separation of ionic surfactant (sodium dodecylbenzenesulfonate and benzalkonium chloride) and non-ionic surfactant(polyoxyethylene octylphenyl ether) using Asahipak GF-310 HQ (a multisolvent GPC column) is shown here. The upper two chromatograms show the separation of two substances, sodium dodecylbenzenesulfonate and polyoxyethylene octylphenyl ether (left) and benzalkonium chloride and polyoxyethylene octylphenyl ether (right). The lower chromatogram shows the separation of three substances, sodium dodecylbenzenesulfonate, benzalkonium chloride and Polyoxyethylene octylphenyl ether.



Sample : 50ppm each, 20micro-L
A. Sodium dodecylbenzenesulfonate
B. Benzalkonium chloride
C. Polyoxyethylene octylphenyl ether

Column       : Shodex Asahipak GF-310 HQ (7.5mmID*300mm)
Eluent       : H2O/CH3CN=50/50
Flow rate    : 0.6mL/min
Detector     : UV(220nm)
Column temp. : 30deg-C