Comparison with ODS Column (1)

The comparison of Asahipak GF-310 HQ ( a multisolvent GPC column ) and an ODS column when they were used for the measurement of POE distribution of octylphenol ethoxylate is shown here. When GF-310 HQ was used, good chromatograms were obtained using 35 and 40% acetonitrile concentration. On the other hand, when an ODS column was used, POE distribution can be measured using 55% acetonitrile concentration but the separation is not sufficient.


Sample :
Octylphenol ethoxylate

Column       : Shodex Asahipak GF-310 HQ (7.5mmID*300mm)
Eluent       : CH3CN/H2O
Flow rate    : 0.6mL/min
Detector     : UV(280nm)
Column temp. : 30deg-C


Column       : ODS column from the other manufacturer
Eluent       : CH3CN/H2O
Flow rate    : 0.8mL/min
Detector     : UV(280nm)
Column temp. : 30deg-C