Mixture of Nonylphenoxy Carboxylic Acids

Each polyoxyethylene chain of nonylphenoxy carboxylic acids was analyzed by gradient elution with RSpak JJ-50 4D.



Sample : 1ppm Mixed nonylphenoxy carboxylic acids, 5micro-L
(Hayashi Pure Chemical Ind.,Co.,Ltd code.990-56063 Nonylphenoxy carboxylic acids mix (n=0 to 9))
1. Nonylphenoxy acetic acid
2. Nonylphenol monoethoxy acetic acid
3. Nonylphonol diethoxy acetic acid
4. Nonylphonol triethoxy acetic acid
5. Nonylphonol tetraethoxy acetic acid
6. Nonylphonol pentaethoxy acetic acid
7. Nonylphonol hexaethoxy acetic acid
8. Nonylphonol heptaethoxy acetic acid
9. Nonylphonol octaethoxy acetic acid
10. Nonylphonol nonathoxy acetic acid

Column       : Shodex RSpak JJ-50 4D (4.6mmID*150mm)
Eluent       : (A) 25mM CH3COONH4 buffer(pH9.5)/CH3CN=85/15
               (B) 25mM CH3COONH4 buffer(pH9.5)/CH3CN=70/30
                Linear gradient; 0min to 75min, (A) to (B) 
Flow rate    : 0.6mL/min
Detector     : Fluorescence Detector(Ex. 225nm, Em. 300nm)
Column temp. : 40deg-C