Effect of Acetonitrile Concentration

Alchohols were analyzed using SUGAR SC1211. By increasing acetonitrile concentration in the eluent, the separation of alcohols becomes insufficient.


Sample :
1. Glycerol
2. Ethyl alcohol
3. n-Propyl alcohol
4. sec-Butyl alcohol
5. Isobutyl alcohol
6. n-Butyl alcohol
7. Isopentyl alcohol
8. n-Pentyl alcohol

Column       : Shodex SUGAR SC1211 (6.0mmID*250mm)
Eluent       : (A); H2O, (B); H2O/CH3CN=80/20, (C); H2O/CH3CN=60/40 
Flow rate    : 1.0mL/min
Detector     : Shodex RI
Column temp. : 70deg-C