Column : Shodex RSpak DE-413 (4.6mmID*150mm) Eluent : (left): CH3CN/H2O=65/35, (center): CH3CN/H2O=80/20, (right): THF/H2O=50/50 Flow rate : 0.6mL/min Detector : UV(254nm) Column temp. : 40deg-C