Preservatives (4) (DE-413)

Food aditives, which have wide polarity range, can be analyzed using RSpak DE-413 (a column for reversed phase chromatography) by simple isocratic mode in a short time.


Sample : 10micro-L
1. Saccharin sodium 0.005%
2. p-Hydroxybenzoic acid 0.005%
3. Sorbic acid 0.02%
4. Benzoic acid 0.02%
5. Methyl paraben, Methyl p-hydroxybenzoate 0.01%
6. Dehydroacetic acid 0.01%
7. Ethyl paraben, Ethyl p-hydroxybenzoate 0.02%
8. Propyl paraben, Propyl p-hydroxybenzoate 0.01%

Column       : Shodex RSpak DE-413 (4.6mmID*150mm)
Eluent       : (0.05M KH2PO4 + 0.1% H3PO4) aq./CH3CN=35/65
Flow rate    : 1.0mL/min
Detector     : UV(210nm)
Column temp. : 40deg-C