Amides (5) (DE-413)

In order to separate hydrophilic compounds like amides, water or 100% buffer solution is used as the eluent, however, such eluent usually shorten the column life of ODS columns. Since the packing material of RSpak DE-413 is chemically stable and has long column life. The stable analysis results can be obtained using DE-413 even when 100% aqueous solution is used as the eluent.


Sample : Amides, 10micro-L
1. Acetamide 100mg/L
2. Propionamide 100mg/L
3. Acrylamide 10mg/L
4. n-Butylamide100mg/L
5. Methacrylamide 10mg/L

Column       : Shodex RSpak DE-413 (4.6mmID*150mm)
Eluent       : 10mM H3PO4 aq.
Flow rate    : 1.0mL/min
Detector     : UV(210nm)
Column temp. : 50deg-C