Analysis of Agricultural Chemicals by LC/MS

Thermally unstable and/or involatile agricultural chemicals which cannot be analyzed by GC or GC/MS can be analyzed by LC/MS.
A semi-micro column RSpak DE-213 was used for the separation.


Sample : 20ng each, 20micro-L
1. Asulam
2. 2-(Methyl-4-chlorophenoxy)-propionic acid, MCPP
3. Thiuram
4. Bensulide, SAP
5. Iprodione
6. Pencycuron

(HPLC)
Instrument   : Agilent 1100
Column       : Shodex RSpak DE-213 (2.0mmID*150mm)
Eluent       : (A): 50mM CH3COONH4 aq.
               (B): CH3CN
               0min to 10min, (B)10% to (B)100%
Flow rate    : 0.2mL/min
Column temp. : 40deg-C

(MS)
Instrument   : Agilent 1100MSD
Mass range   : 105-500(m/z)
Ionization   : Electrospray
Polarity     : Positive, Negative
Fragmentor   : 60V
Nebulizer    : N2(50psi)
Drying gas   : N2(12L/min, 350deg-C)