Effect of Flow Rate (ODP2 HP)

The relations between the theoretical plate number (TPN) and flow rate and also between retention time and flow rate were investigated using ODP2 HP 4D (4.6mm ID x 150mm length). Each sample showed the highest TPN at the flow rate of 0.4mL/min, while efficiency decreased at 0.3mL/min and below due to sample diffusion. Also, each sample showed extremely long retention times at 0.5mL/min and below. Therefore a flow rate of 0.5 – 1.0mL/min is recommended for general analysis using ODP2 HP-4D. Flow rate of 0.1 – 0.2mL/min are recommended for ODP2 HP-2D (2mm ID x 150mm length).


Sample : 5micro-L
Phenol 300ppm
Methyl bezoate 350ppm
Toluene 1000ppm
* Ratio of TPN [%] : TPN at a flow rate of 0.5mL/min is set as 100%.

Column       : Shodex ODP2 HP-4D (4.6mmID*150mm)
Eluent       : H2O/CH3CN=55/45
Detector     : UV(254nm)
Column temp. : 40deg-C