Proteins and Peptides

Using gradient elution, a sample containing proteins and peptides with a variety of molecular weight were separated. Asahipak ODP series provides good reproducibility of elution volume as well as high recovery for analysis of proteins and peptides.

No. Proteins
Peptides		 MW VR(mL)
First
Analysis		 VR(mL)
Second
Analysis		 Recovery
(%)
1 Lys-Bradykinin 1188 4.63 4.64 97
2 Bradykinin 1060 5.73 5.70 92
3 Met-Enkephalin 574 7.00 7.00 97
4 Neurotensin 1673 8.04 8.05 99
5 Leu-Enkephalin 556 8.47 8.48 100
6 Substance P 1348 9.24 9.26 93
7 Bacitracin 1450 11.47 11.48 81
8 Insulin 5750 12.24 12.25 95
9 Insulin B chain 3476 12.87 12.89 91
10 Lysozyme 14300 14.43 14.44 96
11 Mastoparan 1479 15.97 15.98 96
12 Myoglobin 17500 18.17 18.18 83 

Column       : Shodex Asahipak ODP-50 6D (6.0mmID*150mm)
Eluent       : 0.05% TFA aq./CH3CN
               Linear gradient: 80/20 to 50/50, 20min
Flow rate    : 1.0mL/min
Detector     : UV(220nm)
Column temp. : 30deg-C