Recovery of Proteins and Enzyme Activity (SP-825)

The tables show good recovery of IEC SP-825 (a column for strong cation exchange chromatography) for proteins and enzyme activity.

Recovery of proteins
Protein	Recovery (%)
Trypsinogen	93
alpha-Chymotrypsinogen A	99
alpha-Chymotrypsin	106
Myoglobin	96
Lysozyme	96
Ribonuclease A	99
Cytochrome c	100
gamma-Globulin	100

Recovery of enzyme activity
Enzyme	Recovery (%)
Trypsin	90
alpha-Chymotrypsin	102

Sample load : 0.5mg each

Column : Shodex IEC SP-825 (8.0mmID*75mm)
Eluent : (A); 20mM Sodium phosphate buffer(pH6.0)
         (B); (A) + 0.5M NaCl
         Linear gradient: 0min to 1min, (A) to (B)