Recovery of Proteins and Enzyme Activity (CM-825)

The tables show good recovery of IEC CM-825 ( a column for weak cation exchange chromatography ) for proteins and enzyme activity.

Recovery of proteins
Protein	Recovery(%)
alpha Chymotrypsinogen A	90
Myoglobin	97
Ribonuclease A	100
Cytochrome c	101
Lysozyme	99

Recovery of enzyme activity
Enzyme	Recovery(%)
alpha Chymotrypsin	97
Lysozyme	102

Sample load : 0.5mg each

Column : Shodex IEC CM-825 (8.0mmID*75mm)

Eluent : (A); 20mM Sodium phosphate buffer(pH7.0)

         (B); (A) + 0.5M NaCl

         Linear gradient: 0min to 1min, (A) to (B)