Durability against Eluent Composition Change

Since the swelling and shrinking of the packing material of Asahipak NH2P-50 columns is considerably small even when the eluent composition is changed, any mixing ratio of acetonitrile and water can be used with the NH2P-50 columns.

Comparison between before and after testing

Sample	Before testing		After testing
	Elution volume (mL)	Plate number	Elution volume (mL)	Plate number
1. Fructose	7.61	11600	7.56	9800
2. Glucose	9.80	7500	9.72	6200
3. Sucrose	13.86	11700	13.75	9300
4. Maltose	16.78	8300	16.66	6700

Column       : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm)
Eluent       : CH3CN/H2O=75/25
Flow rate    : 1.0mL/min
Detector     : Shodex RI
Column temp. : 30deg-C